Fig. 4: Inhibition of the recruitment of blood-derived macrophages by AnSCs and AnSC-Exos in the lung tissues of BLM-induced PF mice. | Cell Death Discovery

Fig. 4: Inhibition of the recruitment of blood-derived macrophages by AnSCs and AnSC-Exos in the lung tissues of BLM-induced PF mice.

From: Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Fig. 4: Inhibition of the recruitment of blood-derived macrophages by AnSCs and AnSC-Exos in the lung tissues of BLM-induced PF mice.The alternative text for this image may have been generated using AI.

A IF stains of F4/80 (marker for resident macrophages) and CD11b (marker for blood-derived macrophages) in lung tissue sections. B, C Percentages of F4/80+ cells and CD11b+ cells, respectively. D–F Western blot bands and the relative intensities of F4/80 and CD11b, respectively. G, H Relative mRNA levels of F4/80 and CD11b, respectively. Note that both AnSCs and AnSC-Exos effectively inhibited recruitment of blood-derived macrophages but had no significant effect on the number of resident macrophages. I Schematic showing in vitro experimental design: Raw264.7 cells were cultured in the inserts in all groups (without IL4), L929 fibroblasts were cultured in the wells of the third and fourth groups, and AnSC-Exos was added to the wells in the second and fourth groups to induce migration of Raw264.7 cells (the green arrow points the direction of cell migration). J Crystal violet staining of the migrated Raw264.7 cells; scale bar = 200 μm. Note that AnSC-Exos indirectly inhibit the recruitment of macrophages by fibroblasts. Mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 as indicated by Student’s t test; n = 3.

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