Fig. 5: Inhibition of fibroblast-induced macrophage migration by AnSC-Exos-let-7b. | Cell Death Discovery

Fig. 5: Inhibition of fibroblast-induced macrophage migration by AnSC-Exos-let-7b.

From: Antler stem cell exosomes alleviate pulmonary fibrosis via inhibiting recruitment of monocyte macrophage, rather than polarization of M2 macrophages in mice

Fig. 5

A Schematic showing experimental design: Raw264.7 cells were cultured in the inserts of all groups, L929 fibroblasts cultured in the wells of the second, fourth and sixth; let-7b-mimics were added in the wells of the third and fourth groups, and let-7b-inhibitor added in fifth and sixth groups. B The migrated Raw264.7 cells were stained with crystal violet; scale bar = 200 μm. Note that let-7b itself had no significant effect on the migration of Raw264.7 cells but significantly inhibited the L929-induced migration of Raw264.7. C–E Expression levels of CCL7 using ELISA (supernatant) and western blot (L929 cells). Value: Mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 as indicated by Student’s t test; n = 3.

Back to article page