Fig. 1: High hydrostatic pressure (HHP) leads to the devitalization of head and neck cancer cells (HNSCC). | Cell Death Discovery

Fig. 1: High hydrostatic pressure (HHP) leads to the devitalization of head and neck cancer cells (HNSCC).

From: Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

Fig. 1

A Biomass reduction as function of relative crystal violet absorption measurement 72 h after treatment. HHP caused dose-dependent cell death in UT-SCC-14 and HNSCC16/46. At 105 MPa, relative absorption reduced by 18–27%. Cytotoxicity increased at 210 MPa and 315 MPa with a maximum absorption for UT-SCC-14: 2.4 ± 4% (210 MPa) and 1.6 ± 3% (315 MPa). Results shown as means and standard deviations; n = 5. Statistical analysis: two-way ANOVA, Tukey’s multiple comparison post hoc test; *P < 0.05, vs. 0 MPa (UT-SCC-14/HNSCC16/46); #P < 0.05, vs. 105 MPa. B Cell death analysis using Yo-Pro 1 iodide/propidium iodide (Yo-Pro 1/PI) flow cytometry 24 h after treatment with UT-SCC-14. HHP at 105 MPa had no effect on vital, apoptotic, and necrotic cell populations. At 210 MPa, the number of apoptotic and necrotic cells increased significantly, but 27 ± 10% remained viable. At 315 MPa, viable cells reduced to 0.6 ± 0.5%. Values are given as means and standard deviations; n = 18; two-way ANOVA, Tukey’s multiple comparison post hoc test; *P < 0.05, vs. 0 MPa; #P < 0.05, vs. 105 MPa; §P < 0.05, vs. 210 MPa.

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