Fig. 5: Cell cycle analysis confirmed viable cells in the S/G2/M phase following 210 MPa high hydrostatic pressure compared to 315 MPa.

NIR-680 autofluorescent cells were tagged with RFP in the G1 phase and GFP2 in the S/G2/M phase. Following 210 MPa and 315 MPa treatment, viability (red) decreased over time. After 315 MPa treatment, no proliferating cells (green) were observed.