Fig. 7: Chorioallantoic membrane (CAM) assay experimental design. | Cell Death Discovery

Fig. 7: Chorioallantoic membrane (CAM) assay experimental design.

From: Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

Fig. 7

Breeding of the fertilized eggs with automatic rotation started from embryonic development day zero (d 0). Eggs were opened and the CAM was prepared at day 6. On day 7, 1 × 106 cells of the respective head and neck squamous cell carcinoma (HNSCC) cell line were treated with 0, 105, 210, and 315 MPa high hydrostatic pressure (HHP) at 20 °C for 10 min. Following 7 days of incubation and tumor growth, euthanasia and harvesting of the CAM were performed. Near-infrared (NIR) fluorescence was measured, and the CAM tissue was prepared for histology.

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