Fig. 9: HOXA5 promotes SPRY2 transcription by binding to the promoter of SPRY2. | Cell Death Discovery

Fig. 9: HOXA5 promotes SPRY2 transcription by binding to the promoter of SPRY2.

From: TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling

Fig. 9: HOXA5 promotes SPRY2 transcription by binding to the promoter of SPRY2.

A SPRY2 mRNA and protein levels in HOXA5-overexpressed and HOXA5-silenced PCa cells. B SPRY2 promoter reporters were co-transfected with HOXA5 overexpressed plasmid into 293T cells. The SPRY2 promoter activity was determined by a dual luciferase assay kit. C ChIP assay showed the binding of HOXA5 to the promoter of SPRY2 in PCa cells. The decreased cell proliferation (D), cell invasion (E), phospho-ERK expression (F), and percentage of CD133+ stem cell subpopulation (G) caused by HOXA5 overexpression were partially rescued by the transfection with SPRY2-shRNA. *P < 0.05, **P < 0.01. #P < 0.05, ##P < 0.01. Results were presented as mean ± SD.

Back to article page