Fig. 8: Rescue effects of XMU-MP-1 in shHIF-1α cells.
From: YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions
A, B MDCK-parental cells were treated with vehicle (Veh) or 10 μM verteporfin (VP) under 1% O2. MDCK-shHIF-1α cells were treated with Veh or 10 μM XMU-MP-1 (XMU) under 1% O2. After 24 h, MDCK-parental and MDCK-shHIF-1α cells were immunostaining with anti-γH2AX antibody. Scale bar: 40 μm. B γH2AX intensity was analyzed and presented as mean ± SEM. ***p < 0.001. C, D MDCK-shHIF-1α cells were treated with Veh or XMU under normoxia or 1% O2 condition for 36 h, followed by Annexin V staining. Scale bar: 20 μm. D Annexin V positive cell number was analyzed and presented as mean ± SEM. **p < 0.01. All images were obtained using confocal microscope.
