Fig. 3: LINC01189 is downregulated in GC tissues and associated with GC metastasis.

A Expression of LINC01189 was verified in GC cell lines and GES-1 cell line using qPCR. ****P < 0.001, GC cell lines vs. GES-1. B LINC01189 expression was assessed using qPCR in 74 paired GC tissues and adjacent normal tissues. C As analyzed by qPCR, the expression of LINC01189 in GC tissues was found to be significantly lower compared to adjacent normal tissues. ****P < 0.001. GC vs. NT. GC Gastric cancer, NT Normal tissues. D The localization and expression levels of LINC01189 in GC tissues and corresponding adjacent normal tissues (n = 30) were determined using in situ hybridization (ISH) analysis. Scale bar: 100 µm. E1/2 Migration potential of LINC01189 overexpression (E1) or LINC01189 knock-down (E2) in GC cells in a wound healing assay. ****P < 0.001, Control vs. LINC01189; ****P < 0.001, NC-siRNA vs. LINC01189-siRNAp. F1/2, G1/2 Migration (F1/2) and invasion (G1/2) transwell assays of GC cells transfected with LINC01189 overexpression plasmids (F1 & G1), LINC01189-siRNAp (F2 & G2), or the corresponding control for 48 h were conducted. H1/2 White-light (H1) and red fluorescence (H2) images of lung metastases in nude mice (n = 5). I Number of metastatic nodules in the lungs. **P < 0.05, Control vs. LINC01189. J Representative images of H&E and IHC staining with antibodies against MMP2 in metastatic cancer tissue. Scale bar: 100 μm.