Fig. 2: Knockdown of ZNF281 enhances mitochondrial function of HCC cells.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A Measurement of OCR in ZNF281 knockdown HLE cells. B Basal respiration, proton leak, and maximal respiration from (A) were quantified. C Lactate release was determined in ZNF281 knockdown HLE cells. D Measurement of OCR in ZNF281 knockdown Huh7 cells. E Basal respiration, proton leak, and maximal respiration from (D) were quantified. F Lactate release was determined in ZNF281 knockdown Huh7 cells. G Confocal laser scanning microscope detected MitoSox fluorescence in HLE and Huh7 cells (bar = 25 μm). H Left: Flow cytometry analyses of MitoSox fluorescence in HLE or Huh7 cells; Right: statistical analysis of MitoSox fluorescence. *p < 0.05, **p < 0.01, and ***p < 0.001. All experiments were performed at least three times.
