Fig. 8: Increment of mitochondrial biogenesis suppresses ZNF281-mediated EMT, invasion and metastasis of HCC.
From: ZNF281 inhibits mitochondrial biogenesis to facilitate metastasis of hepatocellular carcinoma
A, C Matrigel transwell assays (10×) for cancer cell invasion in ZNF281 and TFAM knockdown HLE and Huh7 cells. B, D Quantification (n = 3; **p < 0.01, and ***p < 0.001) from (A) and (C). E Resected lungs for the groups as indicated. Arrows indicate pulmonary metastatic nodules with macroscopic observation. F Numbers of macroscopic visible pulmonary metastatic nodules. G Representative images of haematoxylin and eosin (H&E) staining for the resected lungs. Scale bars: 500 μm. H Representative phase contrast pictures of HLE clones transfected with shRNAs as indicated. Bar 100 μM. I Western blot showing N-cad and Occludin expression levels in ZNF281 and/or TFAM knockdown HLE cells. J Diagram of the role of ZNF281 in the regulation of mitochondrial biogenesis and function in hepatocellular carcinoma.
