Fig. 2: A feedback loop exists between c-Jun and CNOT3. | Cell Death Discovery

Fig. 2: A feedback loop exists between c-Jun and CNOT3.

From: Targeting the up-regulated CNOT3 reverses therapeutic resistance and metastatic progression of EGFR-mutant non-small cell lung cancer

Fig. 2

A, B PC-9 cells were transfected with a control or CNOT3 siRNA#1. Twenty-four hours after transfection, cells were treated with gefitinib for 24 h. Then cells were subjected to western blotting to assess protein expression levels (A). c-Jun mRNA levels in PC-9 cells were measured by q-PCR (B). C, D PC-9 cells were transfected with a control or CNOT3 siRNA#1. Twenty-four hours after transfection, cells were treated with CHX (10 μM) for the indicated time, then cells were subjected to western blotting to assess protein expression levels (C). Twenty-four hours after transfection, cells were treated with MG132 (1 μM) for 9 h, then cells were subjected to western blotting to assess protein expression levels (D). E Genes that were up-regulated over 1.5-fold in the CNOT3-depleted PC-9 cells were identified using KEGG analysis. F, G PC-9 cells were transfected with a control or CNOT3 siRNA. Twenty-four hours after transfection, cells were treated with MG132 for 9 h. Cell lysates were immunoprecipitated with an anti-c-Jun antibody and analyzed by western blotting with the indicated antibodies (F). Representative images of PC-9 cells stained with anti-c-Jun antibody, ER tracker and DAPI (G). Data are shown as the mean±S.E.M. n = 3. B Student’s t-test.

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