Fig. 3: RPLP2 promotes aerobic glycolysis in HCC cells. | Cell Death Discovery

Fig. 3: RPLP2 promotes aerobic glycolysis in HCC cells.

From: RPLP2 activates TLR4 in an autocrine manner and promotes HIF-1α-induced metabolic reprogramming in hepatocellular carcinoma

Fig. 3

A Lactate production at 24 h in HCC cells after RPLP2 knockdown (n = 3, mean ± SD). B Western blotting was used to detect the expression of glycolysis-related enzymes and glucose transporters, and C, D show the greyscale analysis (n = 3, mean ± SD). E, F Protein expression levels of HIF-1α after RPLP2 knockdown and the greyscale analysis (n = 3, mean ± SD). G, H Effect of RPLP2 on HIF-1α levels in the cytoplasm and nucleus (n = 3, mean ± SD). Knocking down RPLP2 decreased HIF-1α levels in the cytoplasm and nucleus. I Immunostaining of Hep3B cells with antibodies against HIF-1α (red). Knocking down RPLP2 decreased HIF-1α levels in the nucleus. Scale bar, 25 μm. *P < 0.05 versus corresponding control.

Back to article page