Fig. 6: RPLP2 secreted into the extracellular space activates the TLR4/PI3K/AKT cascade. | Cell Death Discovery

Fig. 6: RPLP2 secreted into the extracellular space activates the TLR4/PI3K/AKT cascade.

From: RPLP2 activates TLR4 in an autocrine manner and promotes HIF-1α-induced metabolic reprogramming in hepatocellular carcinoma

Fig. 6

A Zdock software predicted the binding sites of RPLP2 and TLR4. B Co-IP validated that RPLP2 binds to TLR4 in Hep3B cells. C, D Hep3B cells were treated with the TLR4 activator LPS (100 ng/mL) for 24 h, and Western blot and quantitative analyses showed that the expression level of HIF-1α was increased in RPLP2 knockdown cells treated with LPS compared to untreated RPLP2 knockdown cells (n = 3, mean ± SD). E, F Western blotting was used to determine the effect of LPS on HIF-1α levels in the cytoplasm and nucleus and the quantitative analysis (n = 3, mean ± SD). LPS increased HIF-1α levels in the cytoplasm and nucleus. G Immunostaining of Hep3B cells with antibodies against HIF-1α (red). Knocking down RPLP2 led to a decrease in the nuclear expression level of HIF-1α, which was abrogated by LPS. H, I After treatment with LPS, western blotting detected that downstream p-PI3K and p-AKT both rotated compared to RPLP2 knockdown (n = 3, mean ± SD). Scale bar, 25 μm. *P < 0.05 versus corresponding control; ns means nonsignificant.

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