Fig. 4: RMI1 deficiency aggravated IR-induced DNA damage. | Cell Death Discovery

Fig. 4: RMI1 deficiency aggravated IR-induced DNA damage.

From: RMI1 facilitates repair of ionizing radiation–induced DNA damage and maintenance of genomic stability

Fig. 4

A–C HeLa shRMI1 and shControl cells were exposed to indicated doses of irradiation and the DNA damage level was determined using the comet assay. A Representative images of undamaged and damaged cells. Percentages of DNA tail (B) and tail moment (C) were analyzed. D, E HeLa shRMI1 and shControl cells transfected with RMI1-expressing plasmids or those not transfected were irradiated with 4 Gy of γ-rays and then subjected to the comet assay at indicated time points. D Representative images. E Tail moment was quantified and graphed. F Cells subjected to the comet assay were analyzed for RMI1 by Western blot. The level of DNA damage was represented as the mean of three independent experiments, and at least 200 cells were counted. (*P < 0.05, **P < 0.01).

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