Fig. 2: Loss of MT3 affected cell survival in preosteoclasts. | Cell Death Discovery

Fig. 2: Loss of MT3 affected cell survival in preosteoclasts.

From: Metallothionein 3 promotes osteoclast differentiation and survival by regulating the intracellular Zn2+ concentration and NRF2 pathway

Fig. 2

A Relative cell number of Control and MT3-sh1/sh2 BMMs was presented up to 96 h in growth curve. The data are presented as mean (n = 3). *p < 0.001 vs control. B Representative histograms of cell cycle distribution in BMMs were measured by flow cytometry using Propidium iodide. The average percentage of cell numbers in each phase of the cell cycle is shown in the graphs. The data are presented as mean ± SD (n = 4). ns: no significance, vs control. C Control and MT3-sh1/sh2 of BMM or pOC were either untreated or serum/cytokine starved for 4 h. The levels of PARP and Cleaved Caspase 3, which are markers of apoptosis, were assessed using Western blot analysis. Actin served as a loading control.

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