Fig. 4: Change of RANKL-induced ROS and mitochondrial ROS levels by depletion of MT3 expression. | Cell Death Discovery

Fig. 4: Change of RANKL-induced ROS and mitochondrial ROS levels by depletion of MT3 expression.

From: Metallothionein 3 promotes osteoclast differentiation and survival by regulating the intracellular Zn2+ concentration and NRF2 pathway

Fig. 4

A Representative images of intracellular ROS levels in pOCs were measured by DCFH-DA assay followed by RANKL stimulation for 48 h (scale bar = 100 μm) and the percentage of positive cells and the fluorescence intensity on cells of each well were calculated by Hybrid Cell Count Software (Keyence) (n = 5). B The expressions of NRF2, HO-1, and CAT were detected by western blots. Actin served as a loading control. C Representative images of mitochondrial ROS levels in pOCs were measured by MitoSOX Red Mitochondrial Superoxide Indicator, followed by RANKL stimulation for 48 h (scale bar = 100 μm) and the percentage of positive cells and the fluorescence intensity on cells of each well were calculated, respectively (n = 5). D The expressions of pCREB and PGC-1β were detected by western blots. t-CREB and Actin served as loading controls, respectively. The data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs control.

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