Fig. 2: Androgens protect C2C12 cells from senescence induced by sub-lethal concentrations of H2O2.
From: Filamin A cooperates with the androgen receptor in preventing skeletal muscle senescence
a–d C2C12 cells were left untreated or pre-treated with 10 nM R1881 for 4 h. a Cells were challenged with different concentrations (25, 50, and 150 µM) of H2O2 for 36 h. β-gal absorbance was detected by evaluating the absorption at 420 nm of o-nitrophenol. b Cells on coverslips were challenged with 150 µM H2O2, stained and analyzed for F-actin (red) and nuclei with Hoechst (blue). The arrow indicates multinucleated cells. bar, 5 µm. c Multinucleated cells were counted and represented in the graph. d shows the WB of lysates proteins from cells challenged as (b), using the antibodies against the indicated proteins. a, c Means and SDs are shown; n represents the number of experiments. The asterisk (*) indicates P < 0.05.
