Fig. 5: CEP increases oxidative stress levels in gastric cancer cells. | Cell Death Discovery

Fig. 5: CEP increases oxidative stress levels in gastric cancer cells.

From: Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway

Fig. 5

A AGS and HGC27 were treated with CEP (5, 10, and 15 μmol/L) for 48 h. The lactate dehydrogenase (LDH) release from the cells was measured (n = 3). B The mitochondrial membrane potential was measured with JC-1 dye. AGS and HGC27 cells were treated with CEP for 48 h—scale bar: 100 μm. C Intracellular ROS was detected by DCFH-DA staining (green) and DHE staining (red). AGS and HGC27 cells were treated with CEP (5, 10, and 15 μmol/L) for 48 h, and fluorescence images were captured (n = 3). Scale bar: 100 μm. The data were expressed as means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, significantly different from the control group.

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