Fig. 2: piR-YBX1 acts as a tumor suppressor in TNBC in vitro. | Cell Death Discovery

Fig. 2: piR-YBX1 acts as a tumor suppressor in TNBC in vitro.

From: PIWI-interacting RNA-YBX1 inhibits proliferation and metastasis by the MAPK signaling pathway via YBX1 in triple-negative breast cancer

Fig. 2

A The effect of piR-YBX1 on cell proliferation was measured by a CCK8 assay. B A colony formation assay was utilized to measure cell proliferation. Representative pictures (left) and quantitative statistics (right) of colony numbers. C An EdU incorporation assay was used to assess the proliferation ability of TNBC cells. Representative pictures (left) and quantitative statistics (right) of proliferative cells. Scale bar: 100 μm. D, E Transwell and invasion assays were performed to evaluate the ability of migration and invasion abilities of TNBC cells. Representative pictures (left) and quantitative statistics (right) of penetrating cells. Scale bar: 100 μm. F A would healing assay was performed to analyze the migration ability of TNBC cells. Representative pictures (left) and quantitative statistics (right) of the migration rate. Scale bar: 100 μm. G The expression levels of EMT-related markers were analyzed by western blot. The data are showed as the mean ± SD, *P < 0.05 **P < 0.01, ***P < 0.001, ****P < 0.0001. Data were analyzed by (A) two-way ANOVA and (B–F) Students t test.

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