Fig. 2: Silencing MUC1-C induces lipid peroxidation and enhances the effects of SAL treatment.
From: MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cells
A DU-145 cells treated with vehicle or 1 μM SAL for 24 h were analyzed for lipid peroxidation. Shown are histograms (left) and quantitation (mean ± SD of three determinations) (right) of the PE/FITC ratios. B DU-145 cells treated with vehicle or 1 μM SAL for 24 h were analyzed for TfR1 expression by flow cytometry. Listed are the gMFI values. C DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for lipid peroxidation. Shown are histograms (left) and quantitation (mean ± SD of three determinations) (right) of the PE/FITC ratios. D DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days and then incubated with 1 μM SAL for 24 h were analyzed for lipid peroxidation. Shown are histograms (left) and quantitation (mean ± SD of three determinations) (right) of the PE/FITC ratios. E DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days and then incubated with 1 μM SAL for 24 h were analyzed for TfR1 expression by flow cytometry. Listed are the gMFI values. F Lysates from DU-145 cells expressing tet-MUC1shRNA and/or tet-MUC1-C/CD vectors treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. G DU-145 cells expressing tet-MUC1shRNA and/or tet-MUC1-C/CD vectors treated with vehicle or DOX for 7 days and then incubated with 1 μM SAL for 24 h were analyzed for TfR1 expression by flow cytometry. Listed are the gMFI values.
