Fig. 4: MUC1-C induces LRP8 and GPX4 expression.
From: MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cells
DU-145 cells treated with vehicle or 1 μM SAL for 24 h (A) and DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days (B) were analyzed for LRP8 transcripts (left). The results (mean ± SD of 4 determinations) are expressed as relative LRP8 mRNA levels compared to that obtained in vehicle-treated cells (assigned a value of 1). Lysates were immunoblotted with antibodies against the indicated proteins (right). C Genome browser snapshots of ATAC-seq data from the LRP8 gene in DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days (left). Chromatin was analyzed for accessibility by nuclease digestion (right). The results (mean ± SD of 3 determinations) are expressed as % untreated chromatin. D DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were analyzed for LRP8 gene transcription. The results (mean ± SD of 3 determinations) are expressed as relative LRP8 transcription compared to that obtained in vehicle-treated cells (assigned a value of 1). E DU-145/tet-MYCshRNA cells treated with vehicle or DOX for 7 days were analyzed for LRP8 transcripts (left). The results (mean ± SD of 4 determinations) are expressed as relative LRP8 mRNA levels compared to that obtained in vehicle-treated cells (assigned a value of 1). Lysates were immunoblotted with antibodies against the indicated proteins (right). Lysates from DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins (F) and analyzed for GPX activity (G). The results (mean ± SD of 3 determinations) are expressed as relative GPX activity compared to that obtained in vehicle-treated cells (assigned a value of 1). H Lysates from DU-145 cells expressing tet-MUC1shRNA and/or tet-Flag-MUC1-C/CD treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins.
