Fig. 5: FUNDC1 enhanced mitophagy in OGD PC12 cells.
From: FUNDC1-induced mitophagy protects spinal cord neurons against ischemic injury
A–C Co-localization of ATPB with LC3 was observed using laser confocal microscopy to assess mitochondrial autophagy. ATPB was labeled with red fluorescence, LC3 was labeled with green fluorescence, and yellow fluorescence represents the co-localization of ATPB with LC3. All cell nuclei were stained using DAPI (blue). The yellow puncta in each group of cells was quantified (mean ± SD, n = 3). Scale bar = 20 μm. D, E Western blotting analysis of the expression of LC3B and P62 (mean ± SD, n = 3). F–H TEM was used to analyze cellular autophagy. The boxed regions in the upper panels are enlarged in the lower panels. Green arrows: mitochondria; yellow arrows: autophagy; red arrows: mitophagy. Autophagosomes and autophagolysosomes as well as mitochondria in the autophagosomes and autophagolysosomes in each group of cells was quantified (mean ± SD, n = 3). Scale bar = 10 μm or 5 μm. Two-way ANOVA was used to compare the groups. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05.
