Fig. 2: TRIM39 knockout alleviated renal fibrosis induced by UUO. | Cell Death Discovery

Fig. 2: TRIM39 knockout alleviated renal fibrosis induced by UUO.

From: The E3 ubiquitin ligase TRIM39 modulates renal fibrosis induced by unilateral ureteral obstruction through regulating proteasomal degradation of PRDX3

Fig. 2

AThe PCR of gene expression of TRIM39Flox/Flox and Cre Cdh16 mice, gel electrophoresis imaging showed that the length of Cre gene amplification product was 421 bp, and the length of Flox gene amplification product was 226 bp. B, C Representative western blot and quantification images of TRIM39 expression in TRIM39Flox/Flox and TRIM39 conditional knockout (CKO) mice. D, E, H, I Representative western blot and quantification images of α-SMA, collagen I and IV, TNF-α, IL-6 and IL-1β expression in kidneys of the four groups. F Bar graph showing the fold changes of ROS production relative to sham group from three independent samples. G Representative images of H&E, Masson, Sirius Red and DHE staining of kidney tissues of the four groups. The degree of fibrosis was assessed and quantified in five randomly selected microscopic fields. All data were showed as mean ± SEM, Two-way analysis of variance was used. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001. Each experiment was repeated at least three times independently.

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