Fig. 1: ΔNp63α negatively regulates Rac1-GTP in SCC cells.
From: ∆Np63α inhibits Rac1 activation and cancer cell invasion through suppression of PREX1
A JHU-006, A431, and JHU-029 cells were transfected with non-targeting control (NTC) siRNA or p63-targeted siRNA (sip63). B JHU-006 cells were transfected with empty vector (EV) or ΔNp63α-expression plasmid DNA. Whole-cell lysates of transfected cells were immunoprecipitated with p21-activated kinase (PAK) protein binding domain (PBD) followed by immunoblot with Rac1 antibody to detect Rac1-GTP, as described in Materials and Methods. Immunoblot analysis with the indicated antibodies is shown in the bottom panels. β-actin was used as a control to normalize for differences in total protein per lane. The relative abundance of Rac1-GTP was calculated by normalizing the total Rac1 and β-actin signal in the corresponding NTC (A) or EV control (B), and the resulting fold-changes are shown in the bar plots (top). Data are presented as mean ±1 S.E.M. Asterisks indicate P ≤ 0.05 relative to corresponding NTC.
