Fig. 3: ΔNp63α negatively regulates PREX1.
From: ∆Np63α inhibits Rac1 activation and cancer cell invasion through suppression of PREX1
A A431, JHU-006, JHU-029, and FaDu cells were transfected with non-targeting control (NTC) siRNA or p63-targeted siRNA (sip63). 48 h after transfection, PREX1 mRNA was quantified using TaqMan qRT-PCR (top panel). Values were normalized to the NTC control (black bar) and the fold-change (FC) in PREX1 mRNA (gray bar) relative to NTC is shown in the top of panel A. Error bars represent ±1 SD. Statistically significant values (P ≤ 0.05) relative to corresponding NTC are indicated with an asterisk. Immunoblot of ∆Np63α and PREX1 protein are shown in the bottom panel. β-actin was used as a loading control. B H1299 cells were co-transfected with PREX1-Luc reporter plasmid DNA and either empty vector (EV) control or increasing concentrations of ΔNp63α expression plasmid. At 24 h after transfection, a dual luciferase assay was performed in triplicate. Relative luciferase units (RLU) were calculated from the ratio of Firefly luciferase activity to Renilla luciferase activity normalized to EV control. Values are shown as mean ±1 S.E.M. Statistically significant values (P ≤ 0.05) relative to EV controls are indicated with an asterisk. C JHU-006 cells were co-transfected with non-targeting control (NTC) siRNA or p63-targeted siRNA (sip63). After 24 h, cells were transfected with PREX1-Luc reporter plasmid. Following another 24 h, a dual luciferase assay was performed. Relative luciferase units (RLU) were calculated as the ratio of Firefly luciferase activity to Renilla luciferase activity and normalized to NTC control. Values are shown as mean ±S.E.M. Statistically significant values (P ≤ 0.05) relative to NTC controls are indicated with an asterisk. ∆Np63α protein and the β-actin loading control were analyzed by immunoblot (bottom).
