Table 1 Data (mean ± S.E.) related to the electrophysiological properties of SH-SY5Y cells in different culture conditions and days of differentiation.
DIV | INa (pA/pF) | N | IK (pA/pF) | N | Vrest (mV) | N | AP Frequency (Hz) | N | Spontaneous Activity (%) | N | |
|---|---|---|---|---|---|---|---|---|---|---|---|
2D DMAP1 | 5 DIV | 153 ± 25 | 19 | 108 ± 14 | 19 | −28 ± 2 | 20 | 3,6 ± 0,8 | 18 | 0% | 8 |
10 DIV | 57 ± 6 | 13 | 53 ± 6 | 13 | −24 ± 2 | 12 | 1,4 ± 0,4 | 9 | 0% | nd | |
3D Literature | 10 DIV | 115 ± 10 | 33 | 120 ± 7 | 31 | −28 ± 1 | 42 | 2 ± 0,3 | 38 | 0% | 10 |
20 DIV | 114 ± 12 | 5 | 97 ± 13 | 5 | −30 ± 4 | 5 | 3,2 ± 1,4 | 5 | 0% | nd | |
3D DMAP1 Coating | 10 DIV | 215 ± 31 | 21 | 118 ± 11 | 21 | −32 ± 2 | 21 | 3 ± 0,4 | 20 | 0% | 12 |
15 DIV | 218 ± 36 | 10 | 101 ± 15 | 10 | −31 ± 3 | 9 | 3 ± 1 | 9 | 0% | 6 | |
3D DMAP1 Mix | 10 DIV | 187 ± 23 | 13 | 118 ± 19 | 13 | −32 ± 4 | 9 | 3,5 ± 0,5 | 10 | 0% | 7 |
15 DIV | 306 ± 31 | 20 | 141 ± 21 | 20 | −39 ± 2 | 21 | 4 ± 0,6 | 21 | 0% | 5 | |
20 DIV | 273 ± 35 | 12 | 120 ± 6 | 12 | −45 ± 2 | 13 | 5 ± 0,7 | 14 | 7% | 13 | |
25 DIV | 211 ± 93 | 4 | 46 ± 12 | 4 | −39 ± 2 | 3 | 4 ± 1 | 4 | 0% | 3 | |
3D DMAP2 Mix | 10 DIV | 154 ± 32 | 25 | 89 ± 12 | 25 | −24 ± 2 | 29 | 2 ± 0,5 | 15 | 0% | 26 |
20 DIV | 171 ± 45 | 8 | 125 ± 24 | 8 | −34 ± 4 | 12 | 4,5 ± 0,7 | 11 | 12% | 8 | |
25 DIV | 275 ± 30 | 19 | 134 ± 8 | 19 | −37 ± 3 | 18 | 5 ± 0,5 | 16 | 25% | 16 | |
30 DIV | 392 ± 186 | 16 | 102 ± 11 | 16 | −29 ± 2 | 18 | 3,7 ± 0,9 | 14 | 33% | 18 | |
40 DIV | 225 ± 27 | 12 | 85 ± 17 | 12 | −30 ± 1 | 16 | 4 ± 0,8 | 15 | 37% | 16 |
