Fig. 2: Yap cKO eyes exhibit a breakdown of the blood-aqueous barrier.
From: Uveitic glaucoma-like features in Yap conditional knockout mice
A, B Sections from control (Yapflox/flox) and Yap cKO eyes at 2 (2 M, n = 4; A) or 9 months of age (9 M, n = 4; B). Sections were stained with IsoB4 (red) to assess blood vessel wall integrity and with anti-albumin antibody (green) to assess blood leakage from capillaries. Nuclei were counterstained with DAPI (blue). Dashed white lines delineate the iris and the ciliary body. Hashtag points to an intact blood vessel harboring albumin staining exclusively inside the lumen. Regions inside the dashed white squares are depicted at a higher magnification on the right panels. Shown are either fluorescence (a, b, c, d) or brightfield (a’, b’, c’, d’) images. In panels a, a’, arrows point to normal iridal blood vessels. In c, c’, d, d’, asterisks indicate proteinaceous exudates, while arrowheads indicate abnormal blood vessels. CB: Ciliary body, Co: Cornea, I: Iris, L: Lens, R: Retina. Scale bars: left panels, 100 mm; right panels, 20 mm. C Fundus imaging and fluorescein angiography (en-face and profile views) of 9-month-old control (n = 5) and Yap cKO (n = 7) iris. Arrowheads indicate microaneurisms.
