Fig. 4: P-α-syn interacts with and activates TLR2, promoting cellular inflammatory responses in RSC96 cells. | Cell Death Discovery

Fig. 4: P-α-syn interacts with and activates TLR2, promoting cellular inflammatory responses in RSC96 cells.

From: Phosphorylated α-synuclein deposited in Schwann cells interacting with TLR2 mediates cell damage and induces Parkinson’s disease autonomic dysfunction

Fig. 4: P-α-syn interacts with and activates TLR2, promoting cellular inflammatory responses in RSC96 cells.The alternative text for this image may have been generated using AI.

A, B Representative confocal images of TLR2 (green) and p-α-syn (red) co-localization in RSC96 cells stimulated with α-syn PFF for 24 h, and the mean fluorescent density of TLR2 in PBS or α-syn PFF group, scale bar=10 μm, enlarged scale bar=5 μm. C The interaction between p-α-syn and TLR2 was detected in RSC96 cells by Co-IP assay. D–F Representative western blot bands of TLR2 and p-NF-κB /NF-κB, and the quantitative analysis. n = 4. G Representative images of NF-κB nuclear translocation after stimulation of α-syn PFF for 24 h, scale bar=10 μm, enlarged scale bar=5 μm. H–J The mRNA levels of NLRP3, TNF-α, and IL-1β were quantified in RSC96 cells. n = 6. Data were presented as mean ± SEM and analyzed using Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. Cell experiments were repeated three times independently. Co-IP co-immunoprecipitation.

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