Fig. 5: Leukaemia Inhibitory Factor presents anti-metastatic properties in vivo and affects organ colonisation.

A Schematical representation of the in vivo experimental procedure and organs collected. Mice were injected with LIF-treated (green) or not (blue) MKN45 cells (10,000, 100 and 1000). Results obtained from 100 to 1000 cell conditions were grouped and named 100–1000. Metastasis follow-up of B and E Percentage of mice developing metastases; C and F Metastasis signal quantification in vivo; D, G) Liver metastatic signal quantification. Percentages were converted to binary values (0 = negative signal and 1 = positive signal) for statistical analysis. Total flux of bioluminescent signal ± SEM is represented in C, D, F, G. H Percentage of metastasis-bearing mice. 11–16 mice were injected with 100 to 10,000 MKN45-luciferase expressing cells previously treated or not with LIF, and bioluminescence was recorded to detect metastases in the different organs. *p < 0.05 and **p < 0.005 vs. untreated controls, Mann–Whitney statistical analyses.