Fig. 4: MiR-195-5p negatively regulated HSL expression in glioblastoma cells. | Cell Death Discovery

Fig. 4: MiR-195-5p negatively regulated HSL expression in glioblastoma cells.

From: Hsa_circ_0021205 enhances lipolysis via regulating miR-195-5p/HSL axis and drives malignant progression of glioblastoma

Fig. 4

A, B After overexpression of 7 miRNAs predicted by Starbase website, qRT-PCR was performed to evaluate the relative expression of HSL. C The binding sites between miR-195-5p and HSL predicted by Starbase. D, E miR-195-5p targets at HSL verified by dual-luciferase reporter assay. F The expression of miR-195-5p in glioblastoma cell lines (SNB19, LN229, U87MG, U251MG, T98G) by qRT-PCR. G miR-195-5p expression in 18 clinical glioblastoma specimens and 8 peri-tumor brain tissues by qRT-PCR. H Correlation analysis on the relationship between relative mRNA levels of miR-195-5p and HSL in 18 glioblastoma specimens. I The protein expression of HSL after transfection with NC, miR-195-5p mimics, miR-195-5p mimics+vector, or miR-195-5p mimics+HSL, respectively. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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