Fig. 2: Effects of overexpression of miR-204-5p on gADSCs differentiation into hepatocytes. | Cell Death Discovery

Fig. 2: Effects of overexpression of miR-204-5p on gADSCs differentiation into hepatocytes.

From: Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers

Fig. 2

A Glycogen vesicles in hepatocytes detected using glycogen PAS staining (Bar: 50 μm). B ALB in the supernatant of hepatocytes detected using ELISA. C Determination of urea in the supernatant of hepatocytes. D Cellular immunofluorescence identified the expression of hepatocyte marker genes AFP, ALB, HNF4A, and KRT18 before and after differentiation in the Control group (Bar: 100 μm). E Fluorescence intensity analysis of D. F Cellular immunofluorescence identified the expression of AFP, ALB, HNF4A, and KRT18 after differentiation in the Control, LV_Con, and LV_miR groups (Bar: 100 μm). G Fluorescence intensity analysis of F. H Transcription of AFP, ALB, HNF4A, and KRT18 detected using real-time qPCR. Data were presented as mean ± SD; P values were determined by a two-tailed unpaired t test, *P > 0.05, **0.01 < P < 0.05, ***P < 0.01.

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