Fig. 7: Effects of NOTCH signaling pathway on gADSCs differentiation into neurocytes. | Cell Death Discovery

Fig. 7: Effects of NOTCH signaling pathway on gADSCs differentiation into neurocytes.

From: Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers

Fig. 7

A Cell morphology before and after differentiation of the Control, NOTCH(+), and NOTCH(−) groups into neurocytes (Bar: 50 μm). B NGF in the supernatant of neurocytes detected using ELISA. C Cellular immunofluorescence identified the expression of ENO2, TAU, MAP2, and RBFOX3 after differentiation in the Control, NOTCH(+), and NOTCH(−) groups (Bar: 100 μm). D Transcription of ENO2, TAU, MAP2, and RBFOX3 before and after differentiation in the Control, NOTCH(+) and NOTCH(−) groups detected using real-time qPCR. Data were presented as mean ± SD; P values were determined by a two-tailed unpaired t test, *P > 0.05, **0.01 < P < 0.05, ***P < 0.01.

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