Fig. 1: PATJ downregulation after IS associates with favorable functional outcome at 3 months.

PATJ mRNA expression determined by RT-PCR (A, C, D) and by array-based gene expression (B). A Lower PATJ mRNA expression in peripheral blood extracted 24 h after IS in patients with functional independence (mRS < 3, n = 25) compared with patients with marked disability (mRS > 3, n = 25). *p < 0.05 as compared to mRS > 3 (Student’s t-test). B The G risk allele of rs76221407 SNP of PATJ gene confers higher expression in peripheral blood. *p < 0.05 as compared to the AA genotype (Student’s t-test). C Patj expression significantly decay in the ischemic region of mice brain subjected to 1 h transient middle cerebral artery occlusion. Patj mRNA levels were compared between the ipsilateral ischemic region (IL) and the contralateral CTL region (CL) of cortical brain homogenates, measured at 48 h after reperfusion. **p < 0.01 as compared to CL region (Paired t-test). D Oxygen-glucose deprivation (OGD) for 5 and 10 h induces PATJ downregulation in hCMEC/D3 ECs. **p < 0.01; *p < 0.05 as compared to normoxia (Student’s t-test). E PATJ steady-state levels significantly decrease in ECs subjected to HIF-1α-dependent chemical hypoxia. Depletion of the 80 kDa specie of PATJ observed after 4 h incubation with cobalt chloride (CoCl₂). Prolonged incubation for 24 and 48 h accounted for depletion of the 230 and 200 kDa species. GAPDH was used as loading control. F Relative quantification of the integrated band intensities of three different WBs. Bars represent mean intensity ± SD. *p < 0.05; **p < 0.01 as compared to CTL cells (ANOVA followed by Bonferroni’s test).