Fig. 5: PATJ depletion causes nuclear translocation of YAP/TAZ, MUC1-C, β-catenin, and ZEB1 and actin cytoskeleton remodeling. | Cell Death Discovery

Fig. 5: PATJ depletion causes nuclear translocation of YAP/TAZ, MUC1-C, β-catenin, and ZEB1 and actin cytoskeleton remodeling.

From: Role of PATJ in stroke prognosis by modulating endothelial to mesenchymal transition through the Hippo/Notch/PI3K axis

Fig. 5: PATJ depletion causes nuclear translocation of YAP/TAZ, MUC1-C, β-catenin, and ZEB1 and actin cytoskeleton remodeling.The alternative text for this image may have been generated using AI.

A Cytosolic (Cyt) and Nuclear (Nucl) localization of ZEB1, β-catenin, YAP/TAZ, and MUC1-C. Voltage-dependent anion channel (VDAC) and LIM Kinase (LIMK) were used as cytosolic markers and Histone 3 (H3) as nuclear marker. B–F Quantifications of nuclear/cytosolic ratios for YAP (B), TAZ (C), MUC1-C (D), β-catenin (E) and ZEB1 (F). G Immunofluorescence using phalloidin to label F-actin (red) and YAP-antibody (green). H, I PATJ KD decreased the F/G actin ratio. H Representative immunoblot of F-actin and G-actin. I Relative quantification of the F/G actin ratio. Bars represent mean intensity ± SD of three independent experiments. ***p < 0.005 as compared to CTL cells (ANOVA followed by Bonferroni’s test). J WB analysis showing depletion of the actin cytoskeleton markers myosin light chain (MLC) and ARHGAP6 in PATJ KD3 cells.

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