Table 1 Sequences of each assay.
assay | name | sequence |
|---|---|---|
siRNA | si-NC-sense | 5’-UUCUCCGAACGUGUCACGUTT-3’ |
si-NC-antisense | 5’-ACGUGACACGUUCGGAGAATT-3’ | |
si-FOXC1#1-sense | 5’-GCAUCUACCAGUUCAUCAUTT-3’ | |
si-FOXC1#1-antisense | 5’-AUGAUGAACUGGUAGAUGCTT-3’ | |
si-FOXC1#2-sense | 5’-CCUACAACAUGUUCGAGAATT-3’ | |
si-FOXC1#2-antisense | 5’-UUCUCGAACAUGUUGUAGGTT-3’ | |
RT-qPCR | FOXC1-F | 5’-TCACAGAGGATCGGCTTGAACAAC-3’ |
FOXC1-R | 5’-ACAGAGACTGGCTGGAAGGGAAG-3’ | |
CBX7-F | 5’-GCGTGCGGAAGGGTAAAGT-3’ | |
CBX7-R | 5’-GCTTGGGTTTCGGACCTCTC-3’ | |
IGF-1R-F | 5’-CTGCTTAGCTTCCTTGCCTCC-3’ | |
IGF-1R-R | 5’-TTATAAACACGCCACGGCCC-3’ | |
CD44-F | 5’-AGCCCATGTTGTAGCAAACC-3’ | |
CD44-R | 5’-TGAGGTACAGGCCCTCTGAT-3’ | |
CD133-F | 5’-AATGCACCAGCGACAGAAG-3’ | |
CD133-R | 5’-CATTCAAGAGAGTTCGCAAGTC-3’ | |
Chromatin immunoprecipitation | FOXC1-F1 | 5’-AATCTCAGGCGTGTGCACAA-3’ |
FOXC1-R1 | 5’-CTAAGAGCTTAACGCTTCCC-3’ | |
CBX7-F1 | 5’-GAGAAACCCACAAGAGACAC-3’ | |
CBX7-R1 | 5’-ACTGCACCAGCCTTCTTTTC-3’ | |
IGF-1R-F1 | 5’-ACCTCTCCTCCTGCACATTC-3’ | |
IGF-1R-R1 | 5’-TCTCCGCGGTTGTTTTCTTG-3’ |
