Fig. 1: Hyper-uricemic individuals exhibited upregulation of the NLRP3-caspase-1 axis and aggravated cellular senescence. | Cell Death Discovery

Fig. 1: Hyper-uricemic individuals exhibited upregulation of the NLRP3-caspase-1 axis and aggravated cellular senescence.

From: Uric acid–driven NLRP3 inflammasome activation triggers lens epithelial cell senescence and cataract formation

Fig. 1

A Anterior lens capsules (shown by the black arrows) were harvested during cataract extraction and then flat-mounted for NLRP3 staining (with green fluorescence) and caspase-1 staining (with red fluorescence). The positive immunoreactivity is shown by the white arrows (scale bars = 50 μm). sUA serum uric acid, ahUA aqueous humor uric acid. B The number of NLRP3- and caspase-1-positive cells was quantified by calculating the ratio versus cells stained with DAPI. C Immunohistochemistry showed that SA-β-gal labeling (shown by the black arrows) was more distinct in the samples from the hyper-uricemic patients than those from the normo-uricemic patients (scale bars = 50 μm). D Quantitative analysis of the SA-β-gal-positive cells in each field was also performed. Mean ± SD, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001.

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