Fig. 3: Parkin depletion inhibits DXR-induced mitophagy in MCF7. | Cell Death Discovery

Fig. 3: Parkin depletion inhibits DXR-induced mitophagy in MCF7.

From: miR-218-5p and doxorubicin combination enhances anticancer activity in breast cancer cells through Parkin-dependent mitophagy inhibition

Fig. 3

A A Venn diagram illustrates the intersection of four gene lists mitophagy-related (keywords “mitophagy” and “autophagy of mitochondrion”) from public databases AmiGO (Gene Ontology), KEGG and Reactome. The central intersection contains Parkin and SQSTM1 genes. B Total lysates from MCF7 cells transfected with GFP or GFP/shParkin vectors, and then treated with DXR 30 µM for 24 h, were immunoblotted with the indicated antibodies. The graphs show the mitochondrial markers COXII and COXIV protein levels normalised on β-actin that has been used as a loading control. Solid dots represent the value respect to the control condition, from from 6 and 4 (for COXII and COXIV respectively) independent experiments. C–E Level of Parkin mRNA in MCF7, in MCF7 grown as a mammospheres and in MDA-MB-231 after miR-218-5p expression, or shParkin expression in MCF7. Dots represent the mean of Ct of at least 3 technical replicates, from at least 3 (for C), 3(for D) and 4 (for E) independent experiments. S.D. are shown in red. One-way ANOVA (Tukey’s multiple comparison) test was performed for B and C. Unpaired t-test (Welch’s correction) was performed for D and E *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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