Fig. 3: wars-1 KD induces DNA damage. | Cell Death Discovery

Fig. 3: wars-1 KD induces DNA damage.

From: Tryptophanyl-tRNA synthetase-1 (WARS-1) depletion and high tryptophan concentration lead to genomic instability in Caenorhabditis elegans

Fig. 3

a wars-1 KD activates checkpoint signaling through CHK-1pSer345. Upon knocking down wars-1 (right column), CHK-1 phosphorylation at Serine 345 (CHK-1pSer345) was increased. b Zoom-in of the dashed boxes in part a displays the CHK-1pSer345 foci in wars-1 KD compared to control RNAi both in mitotic and pachytene regions. c Replication protein A (RPA) foci formation upon wars-1 KD. Microscopic images of extracted germlines from SSM473 (rpa-1::gfp) worms treated with wars-1 show increased RPA-1::GFP foci formation compared to the control RNAi. The bottom row shows the zoom-in of the dashed squares in the first row, presenting the remarkedly higher number of foci-positive germ cells. d Quantification of RPA-1 foci. The percentage of RPA-1 foci-positive cells in the germline of wars-1 KD was significantly higher than the control (P = 0.0268). An unpaired two-tailed t-test with Welch’s correction was used to compare the two groups. The error bars indicate the standard deviation. For each condition, at least 90 germ cells were counted and analyzed for RPA-1 foci. Scale bars shown in each panel represent 20 μm. (*P < 0.05).

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