Fig. 5: Formation of chromatin bridges upon wars-1 KD. | Cell Death Discovery

Fig. 5: Formation of chromatin bridges upon wars-1 KD.

From: Tryptophanyl-tRNA synthetase-1 (WARS-1) depletion and high tryptophan concentration lead to genomic instability in Caenorhabditis elegans

Fig. 5

a Knocking down wars-1 results in the formation of chromatin bridges in the developing embryos. Arrowheads showing chromatin threads between dividing nuclei. Each image represents a projection of 0.3 μm Z stacks. b Zoom in on four different stages of chromatin bridges; from left to right, different stages of bridge breakage/resolution are shown. c Increased chromatin bridges upon wars-1 KD. Knocking down wars-1 significantly increased the percentage of embryos with chromatin Bridges (****P < 0.0001). d Increased number of chromatin bridges per embryo upon wars-1 KD. Knocking down wars-1 significantly increased the number of embryos with chromatin bridges (****P < 0.0001). As the F-test to compare the variances showed significantly different variances (P < 0.0001), an unpaired two-tailed t-test with Welch’s correction was used to compare the two groups. The scale bars in panels a and b represent 20 μm and 2 μm, respectively. For each condition (wars-1 RNAi and control RNAi), more than 90 embryos were analyzed.

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