Fig. 3: Reprogrammed MΦ and 4-IPP induce myeloblast apoptosis in co-culture experiments. | Cell Death Discovery

Fig. 3: Reprogrammed MΦ and 4-IPP induce myeloblast apoptosis in co-culture experiments.

From: Macrophage migration inhibitory factor blockade reprograms macrophages and disrupts prosurvival signaling in acute myeloid leukemia

Fig. 3

A, B AML cell lines were cultured for 4 days on monolayers of M-, or R-MΦ (A) or monolayers of CM- or R-MΦ (B) and analyzed by flow cytometry for apoptosis with Annexin V staining. A n = 25–38 on 13 different HD; B n = 13–26 on 3 different HD. ★ = HL-60, ■ = MV-4-11, ▲ = NB4, ▼ = OCI-AML3, and ♦ = U937. C Primary myeloblast apoptosis induced after 4–7 days in indicated BM co-culture conditions. n = 6–10. Horizontal lines in A–C panels represent the median apoptosis frequency. **p < 0.005, ***p < 0.0005, ****p < 0.0001.

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