Fig. 4: Macrophage reprogramming reverses myeloblast resistance to venetoclax and midostaurin. | Cell Death Discovery

Fig. 4: Macrophage reprogramming reverses myeloblast resistance to venetoclax and midostaurin.

From: Macrophage migration inhibitory factor blockade reprograms macrophages and disrupts prosurvival signaling in acute myeloid leukemia

Fig. 4

A and B MV-4–11 or C, D HL-60 were cultured in plastic or on indicated macrophage monolayer with increasing concentration of venetoclax for 48 h after which the percentage of live cells was measured by flow cytometry with Annexin V and/or 7-aminoactinomycin D staining. E and F MV-4-11 were cultured in plastic or on indicated macrophage monolayer with increasing concentration of midostaurin for 48 h after which the percentage of live cells was measured by flow cytometry with Annexin V and/or 7-aminoactinomycin D staining. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001 compared to M-macrophages (A, C, E) or to CM-macrophages (B, D, F); n = 3–8 different HD.

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