Fig. 7: MIF inhibition in combination with GM-CSF reduces the proportion of protumoral macrophages in vivo.

A Quantification (mean ± SEM) of numbers of dextran-positive macrophages per 100 µm³ of tumor tissue, analyzed from 3-D z-stacks acquired by intravital multiphoton microscopy, using software analyses tools for surface rendering and size classification as described in the “Methods” section and Fig. 6. Panels right of plot show representative examples of 3-D surface rendering in the red/dextran channel of dextran-positive macrophages classified by size from each treatment group; scale bars = 100 µm. B Flow cytometry analysis was performed on U937 tumors from mice treated as indicated, for (I) % of total CD11b⁺ cells, (ii) % of CD11b⁺ and F4/80⁺ double-positive cells, (iii) and % CD206⁺ M2-like macrophages and (iv) % CD86⁺ M1-like macrophages in the CD11b⁺ population, respectively. Data are shown as the mean ± SEM (n = 4–5 mice per group), *p < 0.05.