Fig. 1: Diminished m6A modification in osteoporosis in the elderly.
From: METTL3-mediated m6A modification increases Hspa1a stability to inhibit osteoblast aging
a Colorimetric assessment of m6A levels in bone samples from elderly osteoporosis patients (n = 10). b qRT-PCR analysis of METTL3, METTL14, FTO, and YTHDC1 expression (n = 10). c Colorimetric evaluation of m6A levels in mouse osteoporotic bone tissue (n = 6). d Western blotting to gauge METTL3 protein levels (n = 6). e Colorimetric analysis of m6A levels in H2O2-induced aging of MC3T3-E1 cells (n = 3). f Identification of senescent cells via β-galactosidase staining (n = 3). g Transmission electron microscopy for mitochondrial morphology (n = 3). h METTL3 protein levels were assessed by Western blotting (n = 3). Data represented as mean ± standard deviation from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t-test.
