Fig. 2: HERC2 deficiency enhances the autophagy pathway. | Cell Death Discovery

Fig. 2: HERC2 deficiency enhances the autophagy pathway.

From: Autophagy dysregulation via the USP20-ULK1 axis in the HERC2-related neurodevelopmental disorder

Fig. 2

A HEK293T cells were transfected with either a negative control siRNA (NC) or a siRNA targeting HERC2. Subsequently, the expression levels of the indicated proteins were assessed by immunoblot analysis. LC3 was detected using an anti-LC3B antibody. Quantification of LC3-I and LC3-II proteins was performed and the data were normalized using clathrin heavy chain (CHC) protein levels as an internal loading control. B Negative control (NC) or HERC2 knocked-down cells were treated with 20 mM NH4Cl for 4 h as indicated. Levels of LC3-II were detected and quantified as in (A). The results are presented as fold changes relative to the control condition. Plots display the mean ± standard error of the mean (SEM). Representative results are shown for experiments that were independently repeated at least three times, and individual data points for each independent experimental repetition are represented as individual dots on the graphs. Significance was determined using unpaired Student’s t-test in (A) and two-way ANOVA with Tukey’s correction for multiple comparisons in (B). Significance levels: *p < 0.05; ***p < 0.001.

Back to article page