Fig. 4: USP20 controls ULK1 stability and regulates autophagy.
From: Autophagy dysregulation via the USP20-ULK1 axis in the HERC2-related neurodevelopmental disorder
A, B HEK293T cells were transfected with either the NC or HERC2 siRNA. A siRNA against USP20 was added under the indicated conditions, and the specified protein levels were analyzed by immunoblot. LC3 was detected using an anti-LC3B antibody. The intensity bands corresponding to ULK1 (A) and to LC3-II (B) were quantified and subsequently normalized using clathrin heavy chain (CHC) protein levels as a loading control. The results are presented as fold changes relative to the control condition. Plots display the mean ± standard error of the mean (SEM). Representative results are shown for experiments that were independently repeated at least three times, and individual data points for each independent experimental repetition are represented as individual dots on the graphs. Significance was determined using two-way ANOVA with Tukey’s correction for multiple comparisons. Significance levels: ns no significance; *p < 0.05; **p < 0.01; ***p < 0.001.
