Fig. 5: Activation of p38 drives upregulation of LC3-II and USP20.
From: Autophagy dysregulation via the USP20-ULK1 axis in the HERC2-related neurodevelopmental disorder
A, B HEK293T were transfected with either a control plasmid (CTL) or a plasmid encoding a constitutively active MKK6 protein (MKK6). Cell lysates were analyzed by immunoblot using the indicated antibodies. LC3 was detected using an anti-LC3B antibody. Phospho-p38 (P-p38) and LC3-II protein levels were quantified and normalized based on total p38 protein levels or CHC protein levels, respectively, in (A). Intensity bands corresponding to HERC2 and USP20 were quantified and normalized based on CHC protein levels in (B). The results are presented as fold changes relative to the control condition. Plots display the mean ± standard error of the mean (SEM). Representative results are shown for experiments that were independently repeated at least three times, and individual data points for each independent experimental repetition are represented as individual dots on the graphs. Significance was determined using unpaired Student’s t-test. Significance levels: ns = no significance; *p < 0.05; ***p < 0.001.
