Fig. 3: Investigating the detailed process of mPT-mediated mitochondrial dysfunction using nFCM. | Cell Death Discovery

Fig. 3: Investigating the detailed process of mPT-mediated mitochondrial dysfunction using nFCM.

From: Nano-flow cytometry unveils mitochondrial permeability transition process and multi-pathway cell death induction for cancer therapy

Fig. 3

A Isolated mitochondria were exposed to varying concentrations of BetA—0, 2, 10, 50, 100, or 200 μM BetA for 2 h. Subsequently, the fluorescence signals of mPTP, \(\Delta\)Ψm, Cyt c, and porin in single mitochondria were detected using nFCM. B Isolated mitochondria were treated with 20 μM BetA for different durations—0, 10, 20, 40, 60, 80, 100, or 120 min. Following treatment, the fluorescence of mPTP, \(\Delta\)Ψm, Cyt c, and porin in single mitochondria were assessed by nFCM. C Isolated mitochondria were exposed to various concentrations of CDDP for 2 h, and the levels of \(\Delta\)Ψm and Cyt c were analyzed at the single-mitochondrion level using nFCM. D, E HeLa cells were treated with different concentrations of CDDP (B) or BetA (C) for 24 h. Mitochondria were then isolated, and the levels of \(\Delta\)Ψm and Cyt c were analyzed at the single-mitochondrion level using nFCM. F Bax/Bak double-knockdown HeLa cells were exposed to varying concentrations of BetA for 24 h. Subsequently, mitochondria were isolated, and the levels of \(\Delta\)Ψm and Cyt c were analyzed using nFCM.

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