Fig. 4: mPT-mediated mitochondrial dysfunction can lead to cell death through multiple pathways. | Cell Death Discovery

Fig. 4: mPT-mediated mitochondrial dysfunction can lead to cell death through multiple pathways.

From: Nano-flow cytometry unveils mitochondrial permeability transition process and multi-pathway cell death induction for cancer therapy

Fig. 4

A, B Fluorescence burst area distribution histogram (A) and normalized fluorescence burst area (B) of AIF protein (i), PNPT1 (ii), and mtDNA content (iii) in isolated mitochondria through single-mitochondrial analysis by nFCM. Isolated mitochondria were treated with 100 μM, 200 μM BetA, and 200 μM CDDP for 2 h, respectively. C Western blot measurement of AIF (i) and PNPT1 (ii) content in mitochondria and supernatant after stimulation with 200 μΜ BetA or CDDP. mtDNA concentration in supernatant was quantified in the unit of ng/μL using 1 μL aliquots of the sample via a SpectraMax QuickDrop Micro-Volume Spectrophotometer (iii). Error bars indicate the mean ± standard error of three independent experiments, and statistical significance was determined using paired t-test analysis. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, and n. s., non-significant.

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