Fig. 1: Designing a tandem-fluorescent reporter to measure efferocytic flux. | Cell Death Discovery

Fig. 1: Designing a tandem-fluorescent reporter to measure efferocytic flux.

From: epHero – a tandem-fluorescent probe to track the fate of apoptotic cells during efferocytosis

Fig. 1

A Amino acid sequence of the epHero reporter. Red, green: sequence encoding the mCherry and eGFP proteins, respectively, downstream of the ubiquitin C promoter. B Schematic of epHero probe. When expressed in apoptotic cells, changes in red and green fluorescence can be used to measure trafficking along the phagosome-lysosome pathway. C Confocal imaging of wild-type, live or apoptotic epHero-transduced THP1 cells. Scale bar 10 µm. D Immunoblots of apoptotic wild-type and epHero-transduced THP1 cell lysates probed for mCherry and eGFP.

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