Fig. 6: The epHero reporter reveals nutrition-mediated changes to corpse uptake and acidification during microglial efferocytosis.
From: epHero – a tandem-fluorescent probe to track the fate of apoptotic cells during efferocytosis
A Quantification of the percentage of phagocytosing cells from serum-fed or -starved microglia co-cultured with epHero bait for up to 24 h. Dark grey: complete medium; light grey: without serum. B mCherry/eGFP ratio from microglia that have phagocytosed cargo after being co-cultured with apoptotic epHero bait for up to 24 h, with or without serum starvation. C Representative histograms from B. D Quantification of the percentage of phagocytosing cells from serum-fed or -starved (and re-stimulated) microglia incubated with epHero apoptotic cells for up to 24 h. E Percentage phagocytosing cells from amino acid-fed or -starved microglia co-cultured with epHero bait for up to 24 h. Dark grey: complete medium; light grey: without amino acids. F mCherry/eGFP ratio from amino acid-fed or starved microglia that have engulfed cargo after being co-cultured with apoptotic epHero bait for up to 24 h. G Representative histograms from F. A, B, D, E, F all data points depict mean ± SEM from n = 3 independent experiments (two-way ANOVA with Šídák’s multiple comparisons test).
